Study of LDL by polyacrylamide gradient gel electrophoresis.
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منابع مشابه
Fractionation of heparin-derived oligosaccharides by gradient polyacrylamide-gel electrophoresis.
Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known ...
متن کاملPolyacrylamide Gel Electrophoresis
Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
متن کاملNeutral polyacrylamide gel electrophoresis.
MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...
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We describe a modified polyacrylamide gel electrophoresis method with which plasma proteins are excellently resolved in 4 h. We made a three-layer discontinuous gradient gel with concentrations of 80,60, and 45 g of polyacrylamide per liter of tris(hydroxymethyl)aminomethane/ethylenediaminetetraacetic acid/borate buffer, pH 9.2. Time and material needed in the preparation of the gel was greatly...
متن کاملLDL particle size by gradient-gel electrophoresis cannot be estimated by LDL-cholesterol/apolipoprotein B ratios.
63. 7. Gunja-Smith Z, Boucek RJ. Collagen cross-linking compounds in human urine. Biochem J 1981;197:759–62. 8. Rosen HN, Dresner-Pollak R, Moses AC, Rosenblatt M, Zeind AJ, Clemens JD, Greenspan SL. Specificity of urinary excretion of cross-linked N-telopeptides of type I collagen as a marker of bone turnover. Calcif Tissue Int 1994;54:26–9. 9. Hanson DA, Weis MA, Bollen AM, Maslan SL, Singer ...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2000
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.44.303